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KMID : 0613820130230030368
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Journal of Life Science
2013 Volume.23 No. 3 p.368 ~ p.376
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Production of Inhibitory Compounds against Helicobacter pylori by Culture Condition of Morus alba cv. Cheongmoknosang Callus
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Cho Young-Je
Cha Won-Seup
Kang Sun-Ae
An Bong-Jeun
Ahn Dong-Hyun
Kim Myung-Uk
Chae Jung-Woo
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Abstract
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The optimal condition for Morus alba cv was an MS culture medium at 27℃ for 20 days. Cheongmoknosang callus showed inhibitory activity against Helicobacter pylori at 1.05 g of wet weight of the cultured callus. The callus formation of Morus alba cv. Cheongmoknosang was influenced by naphthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), 6-benzylaminopurine (BA) and kinetin at concentrations of 2 mg/l. The growth rate of callus was higher than it was when these hormones were mixed with a single hormone. Thus, the optimal condition for direct callogenesis was to incubate with mixture (2,4-D/NAA) of 2 mg/l concentration at 27℃ for 20 days. Moreover, the optimal culture condition of the biomass in the mass production of inhibitory compounds against Helicobacter pylori from Morus alba cv. Cheongmoknosang callus was to incubate in an MS broth (each concentration 1 mg/l of 2,4-D and BA). When Morus alba cv. Cheongmoknosang callus were incubated for 20 days in a bioreactor, Helicobacter pylori inhibition of callus extracts was the highest at a clear zone of 16 mm.
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KEYWORD
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Callus, Morus alba cv. Cheongmoknosang, culture condition, inhibitory activity, Helicobacter pylori
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